Journal: Cell Reports

Article Title: Aryl Hydrocarbon Receptor Contributes to the Transcriptional Program of IL-10-Producing Regulatory B Cells

doi: 10.1016/j.celrep.2019.10.018

Figure Lengend Snippet:

Article Snippet: Negative CD43- Isolation Kit , Miltenyi Biotec , Cat# 130-049-801.

Techniques: Western Blot, Recombinant, Methylation, Adjuvant, Enzyme-linked Immunosorbent Assay, Isolation, cDNA Synthesis, SYBR Green Assay, DNA Library Preparation, Purification, Bicinchoninic Acid Protein Assay, Microarray, Ex Vivo, Generated, Software, Red Blood Cell Lysis, Staining, Lysis

Journal: Microarrays

Article Title: Immune-Signatures for Lung Cancer Diagnostics: Evaluation of Protein Microarray Data Normalization Strategies

doi: 10.3390/microarrays4020162

Figure Lengend Snippet: Principal component analysis (PCA) plot showing the first three principal components of the microarray data set ( a ) unnormalized, ( b ) quantile normalized, ( c ) ComBat-adjusted, and ( d ) DWD-adjusted. Each sphere represents one sample; the color of the sphere indicates the different experimental runs. Run 1 to run 6 is represented by the following colors: run 1 = orange, run 2 = cyan, run 3 = dark blue, run 4 = yellow, run 5 = dark green, run 6 = pink.

Article Snippet: SU8 epoxide-coated glass slides were printed with contact printing technology using a NanoPrint TM LM210 (Dynamic Devices, Wilmington, DE, USA) with 48 SMP2 Stealth Microarray Printing Pins (TeleChem ArrayIt Microarray Division, Sunnyvale, CA, USA).

Techniques: Microarray

Journal: Microarrays

Article Title: Immune-Signatures for Lung Cancer Diagnostics: Evaluation of Protein Microarray Data Normalization Strategies

doi: 10.3390/microarrays4020162

Figure Lengend Snippet: ( a ) Study workflow including the production of 16k protein microarrays, array processing with 200 clinical samples, data pre-processing, data visualization, and statistical analysis of microarray data using BRB-ArrayTools. ( b ) Illustrated microarray processing workflow.

Article Snippet: SU8 epoxide-coated glass slides were printed with contact printing technology using a NanoPrint TM LM210 (Dynamic Devices, Wilmington, DE, USA) with 48 SMP2 Stealth Microarray Printing Pins (TeleChem ArrayIt Microarray Division, Sunnyvale, CA, USA).

Techniques: Microarray

Journal: Microarrays

Article Title: Immune-Signatures for Lung Cancer Diagnostics: Evaluation of Protein Microarray Data Normalization Strategies

doi: 10.3390/microarrays4020162

Figure Lengend Snippet: Principal variance components analysis (PVCA) of the microarray data set ( a ) unnormalized, ( b ) quantile normalized, ( c ) ComBat-adjusted, and ( d ) DWD-adjusted. Contribution to variance was estimated for the following factors: run = experimental runs (run 1 to run 6), type = sample type (cancer or control), sex = female/male, age = age group (0–56, 56–64, 64–70, 70–100 years), smoking = smoking habit (current, never, former smoker), resid = residual weighted average proportion variance. Single factors were investigated as well as combinations of factors.

Article Snippet: SU8 epoxide-coated glass slides were printed with contact printing technology using a NanoPrint TM LM210 (Dynamic Devices, Wilmington, DE, USA) with 48 SMP2 Stealth Microarray Printing Pins (TeleChem ArrayIt Microarray Division, Sunnyvale, CA, USA).

Techniques: Microarray